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KMID : 0903520070500010063
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 1 p.63 ~ p.67
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Protective Effect of Methanolic Extracts from Dendrobium nobile Lindl. on H2O2-induced Neurotoxicity in PC12 cells
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Yoon Mi-Young
Kim Ju-Young
Hwang Ji-Hwan
Cha Mi-Ran
Lee Mi-Ra
Jo Kyung-Jin
Park Hae-Ryong
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Abstract
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The neuroprotective effect of methanolic extracts from Dendrobium nobile Lindl. (DME) against H2O2-induced neurotoxicity in PC12 cells was investigated. The treatment of PC12 cells with various DME concentrations under H2O2 resulted in the induction of protective effect in a dose-dependent manner, as determined by the results of an MTT reduction assay, an LDH release assays, and a morphological assay. Interestingly, we also detected reduction of apoptotic bodies and inhibition of caspase-3 activity by DME in H2O2-induced PC12 cells. These data show that the neuroprotective effect of DME against PC12 cells might be related to the suppression of caspase-3 activation. Therefore, these results suggest that DME could be a new potential candidate as chemotherapeutic agents against neuronal diseases.
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KEYWORD
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Dendrobium nobile Lindl., neuroprotective, oxidative stress, PC12 cells
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